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cyclin b1 gfp  (Addgene inc)


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    Structured Review

    Addgene inc cyclin b1 gfp
    Cyclin B1-mScarlet stable HeLa cell line expressing MAD1-NeoGreen and miRFP670-H2B arrested in mitosis with nocodazole, without adding MG132. Scale bar, 5 μm.
    Cyclin B1 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp%E2%80%93cyclin+b1/pCMX%2FcyclinB1-GFP+(27)+(Plasmid+%2326061)/pmc12547712-386-0-4
    Average 93 stars, based on 7 article reviews
    cyclin b1 gfp - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Coiled-coil interactions drive the ectopic condensation of overexpressed MAD1 to promote mitotic slippage in cancer cells"

    Article Title: Coiled-coil interactions drive the ectopic condensation of overexpressed MAD1 to promote mitotic slippage in cancer cells

    Journal: iScience

    doi: 10.1016/j.isci.2025.113638

    Cyclin B1-mScarlet stable HeLa cell line expressing MAD1-NeoGreen and miRFP670-H2B arrested in mitosis with nocodazole, without adding MG132. Scale bar, 5 μm.
    Figure Legend Snippet: Cyclin B1-mScarlet stable HeLa cell line expressing MAD1-NeoGreen and miRFP670-H2B arrested in mitosis with nocodazole, without adding MG132. Scale bar, 5 μm.

    Techniques Used:

    Cyclin B1-mScarlet stable HeLa cell line expressing MAD1-Neogreen and miRFP670-H2B arrested in mitosis with nocodazole, MG132 was added together with nocodazole right after CDK1 inhibitor washout. Scale bar, 5 μm.
    Figure Legend Snippet: Cyclin B1-mScarlet stable HeLa cell line expressing MAD1-Neogreen and miRFP670-H2B arrested in mitosis with nocodazole, MG132 was added together with nocodazole right after CDK1 inhibitor washout. Scale bar, 5 μm.

    Techniques Used:

    Related Articles

    DNA Profiling:

    Article Title: Hematopoietic PBX-interacting protein is a substrate and an inhibitor of the APC/C–Cdc20 complex and regulates mitosis by stabilizing cyclin B1
    Article Snippet: HA–Cdc20, HA–Cdh1 (Provided by Marc Krishner, Harvard Medical School through Addgene), GFP–cyclin B1 (Provided by Prof. Jonathon Pines through Addgene), H2B-mCherry (kind gift from Dr. Robert Benezra), and Clover-Geminin (1–110) (kind gift from Dr. Michael Lin) were purchased from Addgene. pCMV–Ub and H2B–GFP plasmids were kind gifts from Dr. Maddika Subbareddy (Center for DNA Fingerprinting Diagnosis, Hyderabad, India).

    Biomarker Discovery:

    Article Title: Hematopoietic PBX-interacting protein is a substrate and an inhibitor of the APC/C–Cdc20 complex and regulates mitosis by stabilizing cyclin B1
    Article Snippet: HA–Cdc20, HA–Cdh1 (Provided by Marc Krishner, Harvard Medical School through Addgene), GFP–cyclin B1 (Provided by Prof. Jonathon Pines through Addgene), H2B-mCherry (kind gift from Dr. Robert Benezra), and Clover-Geminin (1–110) (kind gift from Dr. Michael Lin) were purchased from Addgene. pCMV–Ub and H2B–GFP plasmids were kind gifts from Dr. Maddika Subbareddy (Center for DNA Fingerprinting Diagnosis, Hyderabad, India).



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    Image Search Results


    Cyclin B1-mScarlet stable HeLa cell line expressing MAD1-NeoGreen and miRFP670-H2B arrested in mitosis with nocodazole, without adding MG132. Scale bar, 5 μm.

    Journal: iScience

    Article Title: Coiled-coil interactions drive the ectopic condensation of overexpressed MAD1 to promote mitotic slippage in cancer cells

    doi: 10.1016/j.isci.2025.113638

    Figure Lengend Snippet: Cyclin B1-mScarlet stable HeLa cell line expressing MAD1-NeoGreen and miRFP670-H2B arrested in mitosis with nocodazole, without adding MG132. Scale bar, 5 μm.

    Article Snippet: Cyclin B1-GFP is from Addgene (26061), GFP-Mps1 is from Addgene (63702), CENP-A, CENP-C, CENP-T, KNL1, Spc24, Spc25, Nuf2, Mis12 were obtained from Dr. Michael Lampson, HOTag3 was ordered as complementary oligomers (IDT), annealed, and cloned using T4 ligation (NEB M0202S).

    Techniques:

    Cyclin B1-mScarlet stable HeLa cell line expressing MAD1-Neogreen and miRFP670-H2B arrested in mitosis with nocodazole, MG132 was added together with nocodazole right after CDK1 inhibitor washout. Scale bar, 5 μm.

    Journal: iScience

    Article Title: Coiled-coil interactions drive the ectopic condensation of overexpressed MAD1 to promote mitotic slippage in cancer cells

    doi: 10.1016/j.isci.2025.113638

    Figure Lengend Snippet: Cyclin B1-mScarlet stable HeLa cell line expressing MAD1-Neogreen and miRFP670-H2B arrested in mitosis with nocodazole, MG132 was added together with nocodazole right after CDK1 inhibitor washout. Scale bar, 5 μm.

    Article Snippet: Cyclin B1-GFP is from Addgene (26061), GFP-Mps1 is from Addgene (63702), CENP-A, CENP-C, CENP-T, KNL1, Spc24, Spc25, Nuf2, Mis12 were obtained from Dr. Michael Lampson, HOTag3 was ordered as complementary oligomers (IDT), annealed, and cloned using T4 ligation (NEB M0202S).

    Techniques: